Sequencing

• What sequencing parameters should be used for Fixed RNA Profiling gene expression libraries?
• Can I pool Fixed RNA Profiling gene expression libraries with other 10x Genomics libraries for sequencing?
• What Illumina sequencers are supported for Fixed RNA Profiling libraries?

Library Construction

• Why do I need a different sample index plate (Dual Index Kit TT) to make Fixed RNA Profiling Feature Barcode libraries?
• What dual index plate is compatible with the Chromium Fixed RNA Profiling?

Sample Preparation

• Are there any recommendations for working with post-mortem tissue for use with the Fixed RNA profiling assay?
• How should tissue samples be transported from the collection site prior to sample fixation for use in the Fixed RNA Profiling assay?
• Are the cell counting recommendations different for the Fixed RNA Profiling assay?
• Is there any additional guidance for fixing Whole Blood for use with the Fixed RNA Profiling assay?
• Are cell types or tissues with high levels of RNases compatible with the Fixed RNA Profiling assay?
• Are neutrophils and other granulocytes compatible with the Fixed RNA Profiling Assay?

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General

• Is the Fixed RNA Profiling assay compatible with intracellular staining?
• Which thermal cyclers are compatible with the Fixed RNA Profiling assay?

Software

• Is it possible to integrate fixed RNA profiling data with 3' or 5' gene expression data?
• Can I use PDX/xenograft tissue with Fixed RNA Profiling?
• Does Cell Ranger normalize UMI counts based on the coverage of genes in Fixed RNA Profiling?
• How do I use Cell Ranger aggr to aggregate Fixed RNA Profiling datasets?