Questions & Answers
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- How can I optimize my nuclei prep for Single Cell ATAC sequencing?
- How can I assess the quality of my nuclei for Single Cell ATAC Sequencing?
- Can I run samples with low cell viability?
- I want to perform both Single Cell ATAC and Gene Expression profiling, which sample preparation protocol should I use?
- Do I need to dissociate tissues into single cells first before isolating nuclei, or can I use the whole tissue?
- Why is using diluted Nuclei Buffer for resuspending my nuclei important?
- How do I calculate median number of peaks per cell for Cell Ranger ATAC?
- How can I compare scATAC and scRNA-seq data from the same biological sample?
- Does the Cell Ranger ATAC pipeline use static thresholds to call peaks and identify cell barcodes?
- How can I compare results from multiple Cell Ranger ATAC v1.0 runs?
- What annotations are included in the downloadable reference genomes for Cell Ranger ATAC?
- How many UMIs can be detected for each cell by Cell Ranger ATAC?