Questions & Answers
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SupportSingle Cell ATAC
Sample Prep - Nuclei
- Can I sort nuclei for Single Cell ATAC sequencing?
- How do I perform a lysis timeline to optimize my nuclei isolation for Single Cell ATAC sequencing?
- How can I optimize my nuclei prep for Single Cell ATAC sequencing?
- How can I assess the quality of my nuclei for Single Cell ATAC Sequencing?
- Can I run samples with low cell viability?
- I want to perform both Single Cell ATAC and Gene Expression profiling, which sample preparation protocol should I use?
Transposition
Library Construction
Sequencing
Software
- In scATAC-seq, how are the z-scores for transcription factor motif enrichment calculated?
- How can I convert the peak-barcode matrix from Cell Ranger ATAC 1.x to a CSV file?
- How do I calculate median number of peaks per cell for Cell Ranger ATAC?
- How can I compare scATAC and scRNA-seq data from the same biological sample?
- Does the Cell Ranger ATAC pipeline use static thresholds to call peaks and identify cell barcodes?
- What annotations are included in the downloadable reference genomes for Cell Ranger ATAC?