Questions & Answers
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- I'm having trouble accurately counting nuclei. What can I do?
- Can I sort nuclei for Single Cell ATAC sequencing or Single Cell Multiome ATAC + GEX?
- How do I perform a lysis timeline to optimize my nuclei isolation for Single Cell ATAC sequencing?
- How can I optimize my nuclei prep for Single Cell ATAC sequencing?
- Can I run samples with low cell viability for ATAC or Multiome assays?
- Do I need to dissociate tissues into single cells first before isolating nuclei, or can I isolate nuclei directly from the whole tissue?
- [error] Entry 0 in sample_defs are missing input FASTQs
- In scATAC-seq, how are the z-scores for transcription factor motif enrichment calculated?
- How can I convert the peak-barcode matrix from Cell Ranger ATAC 1.x to a CSV file?
- How do I calculate median number of peaks per cell for Cell Ranger ATAC?
- Does the Cell Ranger ATAC pipeline use static thresholds to call peaks and identify cell barcodes?
- What annotations are included in the downloadable reference genomes for Cell Ranger ATAC?