Questions & Answers
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In Situ Gene Expression
Subcellular profiling of targeted features from fresh frozen or FFPE tissues.
- How do I thaw Xenium Decoding Module A and B prior to a Xenium Analyzer run?
- What is the purpose of a Xenium Analyzer Readiness Test and how can I perform it?
- Is internet connectivity required for the Xenium Analyzer?
- How do I predict Xenium Analyzer instrument run time?
- How much data can a Xenium Analyzer store?
- After a Xenium Analyzer run, how should I clean the instrument?
- Are Xenium slides charged or coated?
- Can different panels be run sequentially on the same tissue section?
- Can different panels be selected on the same Xenium Analyzer run?
- Can different tissue types be run on a single Xenium Slide?
- Can signal be generated from probe hybridization events with genomic DNA?
- Can slides be further processed with H&E staining or another stains after a Xenium Analyzer run?
Tissue and Sample Preparation
- Do you have recommendations for sectioning skin for Xenium?
- Do you have recommendations for organoids for Xenium?
- Are alternative autofluorescence quenching protocols compatible with Xenium?
- How can I avoid mRNA degradation during sample preparation and slide processing for Xenium In Situ?
- How do I ship Xenium slides with tissue sections?
- How do I study large tissue sections using Xenium?
Tissue Preparation - FFPE
Tissue Preparation - Fresh Frozen
- Can I substitute MilliQ water for nuclease-free water in the Xenium workflow?
- Can I use 100% Tween 20 diluted to 10% Tween 20 for Xenium?
- Can I use alternative third-party reagents for Xenium?
- Can I use Neo-Clear as an alternative to xylene for Deparaffinization with Xenium In Situ Gene Expression?
- What alternative TE buffers can be used for Xenium?
- Does Xenium Analyzer acquire multiple Z stacks?
- For H&E performed after a Xenium Analyzer run, what imaging requirements are recommended?
- What kind of microscope does the Xenium Analyzer use?
- What is the composition of the Xenium Analyzer imaging buffer?
- How does the optics system work on the Xenium Analyzer?