Question: Can I use pre-stained slides with the Visium CytAssist Spatial Gene Expression assay?
Answer: The Visium CytAssist Spatial Gene Expression assay is compatible with H&E stained FFPE tissues pre-sectioned on glass slides. The assay is also compatible with H&E stained, hardset coverslipped, and imaged slides which we refer to as archived slides in the documentation.
Assay performance will depend on the quality of the RNA in the tissue, which in turn will depend on several factors, including the fixation and embedding method, storage conditions, and age of the slide. We recommend performing an RNA quality assessment after hard coverslip removal from archived slides. Additional details regarding processing archived slides can be found in the Visium CytAssist Spatial Gene Expression for FFPE – Tissue Preparation Guide.
Products: Visium CytAssist for FFPE