Question: What 10x assays are compatible with the Chromium Nuclei Isolation Kit?
Answer: The following assays are compatible with the Chromium Nuclei Isolation Kit are:
|
Assay |
Nuclei Isolation Kit version |
Notes |
|
Single Cell Gene Expression (3’v3.1) |
Chromium Nuclei Isolation Kit with RNase Inhibitor |
Gene Expression only, Feature Barcoding and Cellplex not supported |
|
Single Cell Gene Expression (3’v4) |
Chromium Nuclei Isolation Kit with RNase Inhibitor |
Gene Expression only, Feature Barcoding not supported |
|
Single Cell Immune Profiling (5’v2) |
Chromium Nuclei Isolation Kit with RNase Inhibitor |
Gene Expression only, V(D)J and Feature Barcoding not supported |
|
Single Cell Immune Profiling (5’v3) |
Chromium Nuclei Isolation Kit with RNase Inhibitor |
Gene Expression only, V(D)J and Feature Barcoding not supported |
|
Single Cell Multiome ATAC + Gene Expression |
Chromium Nuclei Isolation Kit with RNase Inhibitor |
|
|
Single Cell Fixed RNA Profiling |
Chromium Nuclei Isolation Kit with RNase Inhibitor |
Nuclei are isolated before fixation |
|
Single Cell ATAC (v2) |
Chromium Nuclei Isolation Kit |
Products: Single Cell Gene Expression, Single Cell Immune Profiling, Single Cell ATAC, Single Cell Multiome ATAC + GEX, Fixed RNA Profiling