Question: Do I need to generate both Multiome ATAC and Multiome Gene Expression libraries, or can I perform one or the other?
Answer: The generation and sequencing of both libraries are necessary for Cell Ranger ARC to call cells accurately. One library can be prepared and evaluated using the Bioanalyzer before preparing the 2nd library. The success in one library(Multiome ATAC for example) does not guarantee success in the corresponding library(Multiome GEX or vice versa).
In cases where only one library is of high quality, it is possible to sequence and analyze only the Gene Expression portion of Multiome using Cell Ranger 4+. It is also possible to analyze only the ATAC portion of Multiome independently using Cell Ranger ATAC. Please contact 10x support by emailing firstname.lastname@example.org for further details.
Products: Single Cell Multiome ATAC+GEX