Question: Which genes does each V(D)J-specific primer map to?
Answer: The V(D)J primer sequences used for enrichment are specifically targeted towards the constant region of the T- or B-cell transcripts. This region is constant across multiple transcripts, which allows us to capture multiple variants with just a few specific primers. We use a nested-PCR enrichment method in order to increase specificity of the amplification product.
Please see the tables below for information on which gene each primer maps to:
Human TCR
| Gene | Outer primer sequence | Inner primer sequence |
| TRA | TGAAGGCGTTTGCACATGCA | AGTCTCTCAGCTGGTACACG |
| TRB | TCAGGCAGTATCTGGAGTCATTGAG | TCTGATGGCTCAAACACAGC |
Human BCR
| Gene | Outer primer sequence | Inner primer sequence |
| IGHA |
CAGGGCACAGTCACATCCT |
GGGAAGTTTCTGGCGGTCA |
| IGHD |
TGCTGGACCACGCATTTGTA | GGTGGTACCCAGTTATCAAGCAT |
| IGHE |
GGTTTTGTTGTCGACCCAGTCT | GTGTCCCAGGTCACCATCAC |
| IGHG |
TTGTCCACCTTGGTGTTGCT | TCCTGAGGACTGTAGGACAGC |
| IGHM |
CATGACGTCCTTGGAAGGCA | CACGCTGCTCGTATCCGA |
| IGLC |
TGTGGGACTTCCACTG | TAGCTGCTGGCCGC |
| IGKC |
TTCTCGTAGTCTGCTTTGCTCAG | GCGTTATCCACCTTCCACTGT |
Mouse TCR
| Gene | Outer primer sequence | Inner primer sequence |
| TRA | CTGGTTGCTCCAGGCAATGG | AGTCAAAGTCGGTGAACAGGCA |
| TRB | TGTAGGCCTGAGGGTCCGT | GGCCAAGCACACGAGGGTA |
Mouse BCR
| Gene | Outer primer sequence | Inner primer sequence |
| IGLC_Group1 | TCAGCACGGGACAAACTCTTCT | TACACACCAGTGTGGCCTT |
| IGLC_Group2 | GCAGGAGACAGACTCTTCTCCA | CAGGCCACTGTCACACCACT |
| IGHA_All | AACTGGCTGCTCATGGTGT | CAGGTCACATTCATCGTGCCG |
| IGHD_All | TGGTGCAAGTGTGGTTGAGGT | GAGGCCAGCACAGTGACCT |
| IGHE_Group1 | TGGTCACTTGGCTGGTGGTG | GCAGGGAAGTTCACAGTGCT |
| IGHE_Group2 | CACTTGGCAGGTGAACTGTTTTCT | CTGTTTGAGATCAGTTTGCCATCCT |
| IGHM_All | AACCTTCAAGGATGCTCTTGGGA | TGCGAGGTGGCTAGGTACTTG |
| IGHG_Group1 | GGACAGGGATCCAGAGTTCCA | CCCTTGACCAGGCATCC |
| IGHG_Group2 | AGGTGACGGTCTGACTTGGC | AGGTCACGGAGGAACCAGTTG |
| IGHG_Group3 | GCTGGACAGGGCTCCATAGTT | GGCATCCCAGTGTCACCGA |
| IGHG_Group4 | GGCACCTTGTCCAATCATGTTCC | AGAAGATCCACTTCACCTTGAAC |
| IGKC_All | ATGTCGTTCATACTCGTCCTTGGT | GAAGCACACGACTGAGGCAC |
Products: Single Cell Immune Profiling