Question: I stored my GEM-RT Products, cDNA, and/or final library for longer than recommended in the User Guide. Can I use them to complete the protocol for sequencing?
Answer: 10x Genomics has tested integrity of samples at different stopping points and provided recommendations for storage conditions and duration in the User Guides. We cannot give recommendations for storage outside these guidelines since longer time points have not been tested. However, if you want to check the integrity of your cDNA or final libraries, you can thaw and check the size distribution and concentration using a Bioanalyzer/Tapestation/Kapa quantification kit. This may give you information to evaluate any degradation that may have taken place.
Products: Single Cell Gene Expression, Single Cell Immune Profiling, Single Cell ATAC, Visium