Question: Can samples be treated with UV light, which is known to cross-link DNA? How would UV treatment affect RNA in the sample and subsequent 10x workflows?
Answer: The impact of UV light on cells will vary based on the duration of exposure, intensity, and wavelength. The effects of UV exposure on cellular RNA has not been tested. Cell viability is known to be negatively affected resulting in higher background and failed runs. https://doi.org/10.1016/j.jphotobiol.2013.06.003
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