Question: What is the sample volume that is loaded onto the Chromium Connect instrument?
Answer: The Chromium Connect can either require 10µl or 32µL of cell suspension per reaction and can be selected in the run setup screen. The Connect can handle the same cell numbers as the manual assay of 500-10,000 cells per channel. The recommended cell stock concentration is 700-1200 cells/ul for accurate counting. Refer to the Chromium Next GEM Automated Single Cell 3ʹ Reagent Kits v3.1 User Guide’s (CG000286) or Chromium Next GEM Automated Single Cell 5ʹ Reagent Kits User Guide (v2 Chemistry) (CG000384) which contain both 10µl or 32µL Cell Suspension Volume Calculator Tables for optimal cell loading guidelines.
Products: Chromium Connect