Questions & Answers
For documentation, software and datasets, please visit
SupportAutomated Single Cell Assays
Automated Library Construction
- Can manually generated cDNA from the Controller or Chromium iX/X be brought onto the Connect for Automated Library Construction?
- Can cDNA samples from the 3’ and 5’ workflows be batched for the Automated Library Construction workflow?
- How much cDNA needs to be carried over onto the system for Automated Library Construction?
General
- What is the Chromium Connect?
- Can the Chromium Connect instrument be used by multiple users?
- Is there a recommendation to place the Chromium Connect in a pre-amp or post-amp room?
- What are the levels of cross-contamination on the Chromium Connect?
- How does the data from the Chromium Connect compare to the manual assay?
Instrument Specifications
- What are the weight/dimensions and power requirements needed for the Chromium Connect instrument?
- Does the Chromium Connect have a HEPA filter?
- Can the HEPA CAP be added to the Chromium Connect after installation?
- Is the Chromium Connect USB or ethernet accessible? Is there wifi connectivity?
- Does the Chromium Connect have a UV lamp?
Supported Applications and Reagents
User Interface
- How do I enter sample information for my experiment on the Chromium Connect?
- What instruction-level should I choose when I set up my Chromium Connect run?
- How do I integrate my data from the Chromium Connect into LIMS?
- I forgot my password and I am now locked out of my account on the Connect. How do I reset my password?
3' and 5' Gene Expression
- What parts of the workflow are included on the Chromium Connect?
- What parts of the continuous workflow on the Chromium Connect require manual intervention?
- What is the sample volume that is loaded onto the Chromium Connect instrument?
- Do I have to run 8 samples at a time on the Chromium Connect, or can I just run one or two?
- Can manual Single Cell 3’ reagents be used with the Chromium Connect?
- Are my Chromium Connect reagents safe at room temperature overnight or over the weekend?
V(D)J Amplification
- What parts of the V(D)J amplification workflow are included on the Chromium Connect?
- Does the V(D)J assay begin with cells or cDNA? What is the cDNA volume that is loaded onto the instrument?
- What parts of the V(D)J amplification workflow on the Chromium Connect require manual intervention?
- When running the V(D)J amplification assay, do I have to run 8 samples at a time, or can I just run one or two?
- Do I need to reload anything before running the 5’ V(D)J amplification assay?
- Which species are supported for V(D)J Amplification?
QC Steps: GEM check, cDNA and library QC
- What happens when I have a wetting/clog failure on the Chromium Connect?
- Are there any safe stopping points in the Chromium Connect workflow?
- Can we run feature barcoding off the remaining Chromium Connect cDNA?
- Can the Chromium Connect start after the post GEM-RT step?
- How much of my cDNA is used for library prep on the Chromium Connect?
- Can I update my SI-PCR cycle selection after the mid-run QC?
Optional Assays: qPCR/Pooling
Maintenance
Troubleshooting
- How do I create a troubleshooting package from the Chromium Connect for Support?
- Can I recover my samples if there is a Chromium Connect instrument failure?
- I made a mistake entering the sample index well on the Chromium Connect. Can I change that during the run?
- Why do I keep getting an error on the Chromium Connect that the barcodes aren’t scanning?