Questions & Answers
For documentation, software and datasets, please visit
SupportAutomated Single Cell Assays
Automated 5' Feature Barcode- Cell Surface Protein
- Can the Chromium Automated Single Cell 5' Feature Barcode Library Construction Kit be used with the Automated Library Construction kit (P/N 1000428) on Connect?
- How much amplified DNA from Feature Barcode can be collected during the automated 5' V2 and automated 5' cDNA workflows?
- How does the Automated 5' Feature Barcode Library Construction become enabled during the Automated 5’ v2 and Automated 5’ cDNA v2 kit selections?
Automated Library Construction
This section is for the Automated Library Construction workflow for the Chromium Connect.
- Can manually generated cDNA from the Controller or Chromium iX/X be brought onto the Connect for Automated Library Construction?
- Can cDNA samples from the 3’ and 5’ workflows be batched for the Automated Library Construction workflow?
- How much cDNA needs to be carried over onto the system for Automated Library Construction?
General
- Are there any additional laboratory equipment needed for use with my Chromium Connect?
- What is the Chromium Connect?
- Can the Chromium Connect instrument be used by multiple users?
- Is there a recommendation to place the Chromium Connect in a pre-amp or post-amp room?
- What are the levels of cross-contamination on the Chromium Connect?
- How does the data from the Chromium Connect compare to the manual assay?
Instrument Specifications
- What are the weight/dimensions and power requirements needed for the Chromium Connect instrument?
- Does the Chromium Connect have a HEPA CAP System Filter filter?
- Can the HEPA CAP be added to the Chromium Connect after installation?
- Is the Chromium Connect USB or ethernet accessible? Is there wifi connectivity?
- Does the Chromium Connect have a UV lamp?
Supported Applications and Reagents
User Interface
- How do I enter sample information for my experiment on the Chromium Connect?
- What instruction-level should I choose when I set up my Chromium Connect run?
- How do I integrate my data from the Chromium Connect into LIMS?
- I forgot my password and I am now locked out of my account on the Connect. How do I reset my password?
3' and 5' Gene Expression
- What parts of the workflow are included on the Chromium Connect?
- What parts of the continuous workflow on the Chromium Connect require manual intervention?
- What is the sample volume that is loaded onto the Chromium Connect instrument?
- Do I have to run 8 samples at a time on the Chromium Connect, or can I just run one or two?
- Can manual Single Cell 3’ reagents be used with the Chromium Connect?
- Are my Chromium Connect reagents safe at room temperature overnight or over the weekend?
V(D)J Amplification
- What parts of the V(D)J amplification workflow are included on the Chromium Connect?
- Does the V(D)J assay begin with cells or cDNA? What is the cDNA volume that is loaded onto the instrument?
- What parts of the V(D)J amplification workflow on the Chromium Connect require manual intervention?
- When running the V(D)J amplification assay, do I have to run 8 samples at a time, or can I just run one or two?
- Do I need to reload anything before running the 5’ V(D)J amplification assay?
- Which species are supported for V(D)J Amplification?
QC Steps: GEM check, cDNA and library QC
- What happens when I have a wetting/clog failure on the Chromium Connect?
- Are there any safe stopping points in the Chromium Connect workflow?
- Can we run feature barcoding off the remaining Chromium Connect cDNA?
- Can the Chromium Connect start after the post GEM-RT step?
- How much of my cDNA is used for library prep on the Chromium Connect?
- Can I update my SI-PCR cycle selection after the mid-run QC?
Optional Assays: qPCR/Pooling
Maintenance
Troubleshooting
- How do I create a troubleshooting package from the Chromium Connect for Support?
- Can I recover my samples if there is a Chromium Connect instrument failure?
- I made a mistake entering the sample index well on the Chromium Connect. Can I change that during the run?
- Why do I keep getting an error on the Chromium Connect that the barcodes aren’t scanning?