Question: Can I do custom oligo conjugation for Feature Barcoding assays?
Answer: Yes, it is possible to conjugate custom oligos to antibodies of interest. There are three options for performing custom conjugation of your antibody of interest:
- Partner with BioLegend’s conjugation experts in designing and creating custom oligo-conjugated antibodies - contact your local BioLegend rep for more information on custom conjugation services.
- Perform your own custom conjugation using the 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® from Abcam. This kit contains all the reagents necessary for performing custom conjugation, including oligos compatible with our 5' assay.
- You can also use the universal Oligonucleotide Conjugation Kit from Abcam (previously the Thunder-Link kit from Expedeon), compatible with both our 3' and 5' assays. This kit requires procurement of your own custom oligos for conjugation. Please refer to the 10x Demonstrated Protocol Cell Surface Protein Labeling for Single Cell RNA Sequencing Protocols for guidance on using this kit. A set of 10,000 recommended barcodes distinct from any commercial partners' barcodes is also available at the link above.
Products: Single Cell Gene Expression, Single Cell Immune Profiling, Fixed RNA Profiling Gene Expression