Question: Does Cellranger DNA support multi-species (barnyard) experiments?
Answer: No. Creating a library from a mixture of cells from two different species is colloquially referred to as a "barnyard" experiment. The purpose is to assess the rate of capturing multiple cells in a single partition (GEM) by mixing a 1:1 ratio of cells from two different species, typically human and mouse.
While it is possible to create a Chromium Single Cell DNA barnyard library, the current version of the analysis pipeline (Cell Ranger DNA version 1.0) does not currently support analyzing such data, for example from a xenograft sample.
The multiplet rate of the Single Cell DNA product has been characterized and can be found in the Chromium Single Cell DNA User Reagent Kits User Guide.