Question: Are RNase inhibitors required in the preparation of my sample?
Answer: We recommend using RNase inhibitors when preparing cells samples that have high levels of RNases (such as granulocytes) and other inhibitory compounds, as detailed in our Best practices for working with granulocytes.
For nuclei samples, it is mandatory to use RNAse inhibitor in sample prep. If using the Chromium Nuclei Isolation Kit, we recommend following the user guide chapter corresponding to the downstream assay. For alternative nuclei isolation protocols - if using the 3'GEX assay, we recommend a concentration of 0.2U/ul and for the Multiome assay, we recommend a concentration of 1U/ul in all buffers.
In our demonstrated protocols we have tested Protector RNase inhibitor (Sigma Aldrich PN-3335399001) and found that it gives the best results. Note that some RNAse inhibitors may negatively affect your experiment. Please see: Can I use an alternative RNase inhibitor part number?
Products: Single Cell Gene Expression, Single Cell Multiome ATAC + GEX