Question: Are nuclei samples compatible with CRISPR screening?
Answer: Nuclei samples are not supported with the Single Cell 3’ or 5’ CRISPR Screening assays. In our limited in-house tests using nuclei instead of cells with the 10x Single Cell 3' v3 CRISPR Screening Assay, we were unable to obtain satisfactory data from 10x CRISPR libraries prepared from nuclei. This may be because the sgRNA levels within the nucleus are not high enough for reliable detection in the 10x CRISPR assay. We have not performed any in-house tests with nuclei with the 3’v3.1, 3’v4, 5’v2 or 5’v3 CRISPR Screening assays. For customers interested in running nuclei CRISPR screening, we highly recommend conducting a pilot test.
Products: Single Cell Gene Expression, Single Cell Immune Profiling