Question: Are OCT-embedded samples compatible with the Single Cell Multiome ATAC + Gene Expression assay?
Answer: We have conducted limited testing of OCT-embedded samples with the Single Cell Multiome ATAC + Gene Expression assay. Data quality is likely to vary based on the quality of input tissue. A pilot study is highly recommended.
Nuclei were isolated with the Chromium Nuclei Isolation Kit from 50µm sections of human brain without trimming excess OCT. The Chromium Nuclei Isolation Kit is the only nuclei isolation protocol that has been validated on OCT-embedded tissues in the Single Cell Multiome ATAC + Gene Expression assay.
A summary of our findings comparing flash-frozen and OCT-embedded samples is presented below. Similar cell types were identified in nuclei isolated from both conditions. Further information can be found in the following App Note: Multiomic single nuclei analysis of OCT brain samples informs spatial deconvolution.
Products: Single Cell Multiome ATAC+GEX