What options are there for performing a targeted enrichment for my gene expression libraries?

Question: What options are there for performing a targeted enrichment for my gene expression libraries?

Answer: Depending on the goals for performing a targeted enrichment to enrich for specific transcripts, there are a few options available.

If you are interested in enhancing detection of specific transcripts:

• For 3’ Gene Expression, a custom PCR enrichment can be performed:
  • How do I design a custom PCR-based target enrichment assay for Single Cell 3'?
• Custom PCR enrichment is also possible for VDJ enrichment:
  • How do I design a custom PCR-based target enrichment assay for Single Cell V(D)J?

If you are interested in reducing sequencing costs:

• The Chromium Gene Expression Flex assay offers enhanced gene expression sensitivity. This assay has a recommended minimum sequencing depth of 10,000 reads per cell compared to the 3’ Gene Expression or 5’ Immune Profiling assays which have recommended minimum sequencing depths of 20,000 reads per cell.
• Using JumpCode Genomics’ CRISPRclean kit can reduce unwanted or irrelevant genes to enhance relevant data detection and reduce sequencing burden.

Products: Single Cell Gene Expression, Single Cell Immune Profiling, Single Cell Gene Expression Flex