Feature Barcode - Barcode Enabled Antigen Mapping (BEAM)
- Can I titrate the ratio of biotinylated antigen to BEAM Conjugate to improve the detection of antigen-positive cells in a BEAM-Ab experiment?
- Can I use two fluorophores to identify antigen-positive cells in a BEAM experiment?
- Is BEAM compatible with cell hashing?
- How can I reduce background PE signal for the BEAM-Ab Negative Control Assembly?
- Which antibodies should I include in my fluorescent antibody panel for BEAM flow sorting?
- Can I use more than one MHC allele in a BEAM experiment?
- What are the requirements for peptides for BEAM-T?
- What is the purpose of the quenching step in the BEAM assay?
- What are the requirements for antigens for BEAM-Ab?
- Should I include negative controls in the BEAM assay?
- How do I pre-screen antigens or peptides to check their quality for BEAM?
- How many MHC monomers are present in the final BEAM-T Assembly?
- How many cells should I use as input into BEAM labeling and flow sorting?
- Is flow sorting required for BEAM? Can bead-based enrichment be performed instead?
- Is it necessary to sequence the Gene Expression library in a BEAM experiment?
- What resources are available for getting started with BEAM?
- Is bead-based pre-enrichment of B or T cells recommended for BEAM?
- What sample types are compatible with BEAM?
- What species are supported for use with BEAM?
- Which MHC alleles can be used with BEAM?
- How many antigens or peptides can be tested in each BEAM experiment?
- Why was the part number of the 5’ Feature Barcode Kit updated from 1000256 to 1000541?