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  1. 10X Genomics
  2. Single Cell Immune Profiling
  3. Feature Barcode - Barcode Enabled Antigen Mapping (BEAM)

Feature Barcode - Barcode Enabled Antigen Mapping (BEAM)

  • Which antibodies should I include in my fluorescent antibody panel for BEAM flow sorting?
  • Can I use more than one MHC allele in a BEAM experiment?
  • What are the requirements for peptides for BEAM-T?
  • What is the purpose of the quenching step in the BEAM assay?
  • What are the requirements for antigens for BEAM-Ab?
  • Should I include negative controls in the BEAM assay?
  • How do I pre-screen antigens or peptides to check their quality for BEAM?
  • How many MHC monomers are present in the final BEAM-T Assembly?
  • How many cells should I use as input into BEAM labeling and flow sorting?
  • Is flow sorting required for BEAM? Can bead-based enrichment be performed instead?
  • Is it necessary to sequence the Gene Expression library in a BEAM experiment?
  • What resources are available for getting started with BEAM?
  • Is bead-based pre-enrichment of B or T cells recommended for BEAM?
  • What sample types are compatible with BEAM?
  • What species are supported for use with BEAM?
  • Which MHC alleles can be used with BEAM?
  • How many antigens or peptides can be tested in each BEAM experiment?
  • Why was the part number of the 5’ Feature Barcode Kit updated from 1000256 to 1000541?
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