Library Construction
Frequently asked questions related to steps detailed in the Visium CytAssist Spatial Gene Expression User Guide
- Is it expected to have tissue remaining on the slide after CytAssist-enabled RNA Digestion & Tissue Removal?
- How do I assemble and disassemble the Visium Slide Cassette?
- Why does my Visium CytAssist Spatial Gene Expression library have additional, larger peaks in the trace?
- What does a good Visium CytAssist Spatial Gene Expression library look like?
- What’s the expected range of Cq values that should be obtained when performing the Cycle Number Determination step with the Visium CytAssist Spatial Gene Expression assays?
- What off-slide workflow steps are performed for generating Visium CytAssist Spatial Gene Expression libraries?
- How long does a Visium CytAssist Spatial Gene Expression Sample Run take once the instrument is loaded?
- Do the tissue permeabilization conditions need to be optimized before loading the tissue slides on the Visium CytAssist instrument?
- Are there any time sensitive steps when performing a sample run on the Visium CytAssist instrument?