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  1. 10X Genomics
  2. Visium Spatial Gene Expression for FFPE Assays
  3. Visium CytAssist Spatial Gene Expression
  4. Library Construction

Library Construction

Frequently asked questions related to steps detailed in the Visium CytAssist Spatial Gene Expression User Guide

  • Is it expected to have tissue remaining on the slide after CytAssist-enabled RNA Digestion & Tissue Removal? 
  • How do I assemble and disassemble the Visium Slide Cassette? 
  • Why does my Visium CytAssist Spatial Gene Expression library have additional, larger peaks in the trace?
  • What does a good Visium CytAssist Spatial Gene Expression library look like?
  • What’s the expected range of Cq values that should be obtained when performing the Cycle Number Determination step with the Visium CytAssist Spatial Gene Expression assays?
  • What off-slide workflow steps are performed for generating Visium CytAssist Spatial Gene Expression libraries?
  • How long does a Visium CytAssist Spatial Gene Expression Sample Run take once the instrument is loaded?
  • Do the tissue permeabilization conditions need to be optimized before loading the tissue slides on the Visium CytAssist instrument?
  • Are there any time sensitive steps when performing a sample run on the Visium CytAssist instrument?
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