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  1. 10X Genomics
  2. CytAssist Spatial Gene Expression
  3. Tissue Preparation - FFPE

Tissue Preparation - FFPE

Content related to fixation, embedding, sectioning included in the Visium CytAssist Spatial Gene Expression for FFPE - Tissue Preparation Guide

  • How do I transport my tissue slide to another facility when tissue has already been placed?
  • How should I fix and embed my tissue for Visium CytAssist for FFPE?
  • How is tissue preparation similar and different between Visium for FFPE (v1) and Visium CytAssist for FFPE?
  • What key steps are similar and different when running archived, hard coverslipped slides as compared to freshly placed FFPE sections?
  • What section thickness would we recommend to use with the Visium CytAssist for FFPE solution?
  • How do I determine if my tissue block or archived section will be compatible with the Visium CytAssist for FFPE assay?
  • If the tissue is much larger than the Capture Area, how can they ensure that the area of interest within the tissue is going to generate data?
  • How can I more easily place tissues within the allowable area?
  • What steps can customers take to confirm FFPE tissue quality prior to proceeding with the workflow?
  • What tissue types are compatible with the Visium CytAssist Spatial Gene Expression solution?
  • What happens if a customer operates a run when the tissue lies outside the allowable area on the plain glass slide?
  • Are there any requirements regarding tissue location on glass slides for CytAssist enabled assays?
  • What staining methods are compatible with the Visium CytAssist for FFPE solution?
  • What fixation and embedding methods are compatible with the Visium CytAssist for FFPE solution?
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