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  1. 10X Genomics
  2. Fixed RNA Profiling
  3. Sequencing

Sequencing

  • Can Fixed RNA Profiling libraries be pooled with other dual indexed 10x libraries?
  • Why was the percentage of PhiX for NovaSeq increased for Multiplexed Fixed RNA Profiling libraries sequenced on NovaSeq?
  • Why are the "Reads Mapped Confidently to the Probe Set" or "Reads Mapped Confidently to the Filtered Probe Set" metrics in my Fixed RNA Profiling web summary file low?
  • What sequencing parameters should be used for Fixed RNA Profiling gene expression libraries?
  • Can I pool Fixed RNA Profiling gene expression libraries with other 10x Genomics libraries for sequencing?
  • What Illumina sequencers are supported for Fixed RNA Profiling libraries?
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