Sample Preparation
- Can dissociated FFPE samples be flow sorted for use with the Fixed RNA Profiling assay?
- Does the FFPE Demonstrated Protocol produce a suspension of single nuclei, single whole cells or a mixture of both?
- Can I use Flow Sorting with fixed whole blood samples?
- What QC is available before, during, and after running the Flex Gene Expression assay with FFPE samples?
- Can I use OCT embedded fresh frozen tissue with the Flex Gene Expression assay?
- Can formaldehyde from alternative vendors be used for sample fixation in the Flex Gene Expression assay?
- Can I use formalin fixed tissue as input for the Flex Gene Expression assay?
- What are the validated stopping points for sample storage in the Flex Gene Expression assay?
- How does data generated from Tissue Fixation & Dissociation (Chop/Fix) and Sample Preparation from FFPE Tissue Sections Demonstrated Protocols compare?
- How can I improve my cell/nuclei recovery using the Flex Gene Expression Assay?
- What are best practices for working with organoids in the Flex Gene Expression assay?
- Is there any additional guidance for fixing Whole Blood for use with the Flex Gene Expression assay?
- Are neutrophils and other granulocytes compatible with the Flex Gene Expression assay?
- Can post-fixation samples be sorted in the Flex Gene Expression assay?
- What are the sample quality recommendations for optimal performance of the Flex Gene Expression assay?
- Which tissue dissociation protocols are supported for use with the Flex Gene Expression assay?
- Can alternative fixation buffers or fixation protocols be used for sample fixation with the Flex Gene Expression assay?