Sample Preparation
- What QC is available before, during, and after running the Chromium Gene Expression Flex assay with FFPE samples?
- Can I use OCT embedded fresh frozen tissue with the Flex/Fixed RNA Profiling assay?
- Can formaldehyde from alternative vendors be used for sample fixation in the Fixed RNA Profiling Assay?
- Can I use formalin fixed tissue as input for the Gene Expression Flex assay?
- Can an FFPE tissue slide be used as input for the Isolation of Cells from FFPE Tissue Sections Demonstrated Protocol (CG000632)?
- Can I use Xylene alternatives for Tissue Deparaffinization during the Isolation of Cells from FFPE Tissue Sections Demonstrated Protocol (CG000632)?
- What are the validated stopping points for sample storage in the Fixed RNA Profiling assay?
- Why was the -20°C optional sample storage recommendation removed from the Fixed RNA Profiling assay?
- How does data generated from Tissue Fixation & Dissociation for Chromium Fixed RNA Profiling (Chop/Fix) and Isolation of Cells from FFPE Tissue Sections for Chromium Fixed RNA Profiling (scFFPE) Demonstrated Protocols compare?
- How can I improve my cell/nuclei recovery using the Fixed RNA Profiling Assay?
- How should I prepare my Liberase TH solution for the Isolation of Cells from FFPE Tissue Sections for Chromium Fixed RNA Profiling Demonstrated Protocol?
- How should I prepare my FFPE block upstream of the Isolation of Cells from FFPE Tissue Sections for Chromium Fixed RNA Profiling Demonstrated Protocol?
- What sample types are compatible with the Isolation of Cells from FFPE Tissue Sections for Chromium Fixed RNA Profiling Demonstrated Protocol?
- What block QC is available prior to the Isolation of Cells from FFPE Tissue Sections for Chromium Fixed RNA Profiling Demonstrated Protocol?
- How should I transport my FFPE curls for Isolation of Cells from FFPE Tissue Sections for Chromium Fixed RNA Profiling Demonstrated Protocol?
- What is the range of compatible cell sizes for Fixed RNA Profiling?
- What are best practices for working with organoids in the Fixed RNA Profiling Assay?
- Are there any recommendations for working with post-mortem tissue for use with the Fixed RNA Profiling assay?
- How should tissue samples be transported from the collection site prior to sample fixation for use in the Fixed RNA Profiling assay?
- Are the cell counting recommendations different for the Fixed RNA Profiling assay?
- Is there any additional guidance for fixing Whole Blood for use with the Fixed RNA Profiling assay?
- Are cell types or tissues with high levels of RNases compatible with the Fixed RNA Profiling assay?
- Are neutrophils and other granulocytes compatible with the Fixed RNA Profiling Assay?
- Can post-fixation samples be sorted in the Fixed RNA Profiling assay?
- What are the sample quality recommendations for optimal performance of the Fixed RNA Profiling assay?
- Which tissue dissociation protocols are supported for use with the Fixed RNA Profiling assay?
- Which nuclei isolation protocols are supported with the Fixed RNA Profiling assay?
- Can alternative fixation buffers or fixation protocols be used for sample fixation with the Fixed RNA Profiling assay?
- What cell filters or strainers are recommended for use with Fixed RNA Profiling assay?