.
Logo
  • PRODUCTS
  • TECHNOLOGY
  • COMPANY
  • CAREERS
  • Support
  • COMMUNITY
  • Sign in
  1. Support
  2. Q&A
Q&A NEW CONTACT SUPPORT
  1. 10X Genomics
  2. Fixed RNA Profiling
  3. Sample Preparation

Sample Preparation

  • What are the validated stopping points for sample storage in the Fixed RNA Profiling assay?
  • Why was the -20°C optional sample storage recommendation removed from the Fixed RNA Profiling assay?
  • How does data generated from Tissue Fixation & Dissociation for Chromium Fixed RNA Profiling (Chop/Fix) and Isolation of Cells from FFPE Tissue Sections for Chromium Fixed RNA Profiling (scFFPE) Demonstrated Protocols compare? 
  • How can I improve my cell/nuclei recovery using the Fixed RNA Profiling Assay?
  • How should I prepare my Liberase TH solution for the Isolation of Cells from FFPE Tissue Sections for Chromium Fixed RNA Profiling Demonstrated Protocol?
  • How should I prepare my FFPE block upstream of the Isolation of Cells from FFPE Tissue Sections for Chromium Fixed RNA Profiling Demonstrated Protocol?
  • What sample types are compatible with the Isolation of Cells from FFPE Tissue Sections for Chromium Fixed RNA Profiling Demonstrated Protocol?
  • What block QC is available prior to the Isolation of Cells from FFPE Tissue Sections for Chromium Fixed RNA Profiling Demonstrated Protocol?
  • How should I transport my FFPE curls for Isolation of Cells from FFPE Tissue Sections for Chromium Fixed RNA Profiling Demonstrated Protocol?
  • What is the range of compatible cell sizes for Fixed RNA Profiling?
  • What are best practices for working with organoids in the Fixed RNA Profiling Assay?
  • Are there any recommendations for working with post-mortem tissue for use with the Fixed RNA profiling assay?
  • How should tissue samples be transported from the collection site prior to sample fixation for use in the Fixed RNA Profiling assay?
  • Are the cell counting recommendations different for the Fixed RNA Profiling assay?
  • Is there any additional guidance for fixing Whole Blood for use with the Fixed RNA Profiling assay?
  • Are cell types or tissues with high levels of RNases compatible with the Fixed RNA Profiling assay?
  • Are neutrophils and other granulocytes compatible with the Fixed RNA Profiling Assay?
  • Can post-fixation samples be sorted in the Fixed RNA Profiling assay?
  • What are the sample quality recommendations for optimal performance of the Fixed RNA Profiling assay?
  • Which tissue dissociation protocols are supported for use with the Fixed RNA Profiling assay?
  • Which nuclei isolation protocols are supported with the Fixed RNA Profiling assay?
  • Can alternative buffers or fixation protocols be used for sample fixation?
  • What cell filters or strainers are recommended for use with Fixed RNA Profiling assay?
footer-logo

Biology at True Resolution

About
  • Solutions
  • Technology
  • Company
  • Careers
  • Support
  • Community
  • Contact Us
Legal Notices
  • Privacy Policy
  • Terms of Use
  • Email Preferences
  • Other Legal Notices
Resources
  • Publications
  • Applications
  • Videos
Headquarters
  • +1 925 401 7300
  • +1 800 709 1208
  • info@10xgenomics.com
Social
©2018 10x Genomics. All Rights Reserved.
Sign up for product and event updates