General
- What Fixed RNA Profiling applications are compatible with TotalSeq™️ antibodies?
- When should I start to incorporate the thermal cycler protocol adjustment (updating Pre-Amplification annealing temperature)?
- What happens if I forget to update my thermal cycler program for Pre-Amplification and I perform Barcode Oligo Capture protocols?
- Why is the annealing temperature during Pre-Amplification updated from 67°C to 63°C across all Fixed RNA Profiling protocols?
- Why isn’t TotalSeq™️-B compatible with Fixed RNA Profiling for Multiplexed Samples?
- What 10x kits are required for performing Fixed RNA Profiling with Feature Barcode technology for Protein using Barcode Oligo Capture (TotalSeq™️-C compatible)?
- What does “Barcode Oligo Capture” mean in the context of Feature Barcode Technology with Fixed RNA Profiling?
- Can I pool less than 4 or 16 samples when using the Chromium Fixed RNA Profiling Reagent Kits for Multiplexed Samples?
- What is the maximum number of cells that can be targeted per barcode when using the Chromium Fixed RNA Profiling Reagent Kits for Multiplexed Samples?
- Can I ship fixed single cell/nuclei samples for use with the Fixed RNA Profiling Assay?
- Can I use fewer than the minimum recommended number of cells/nuclei during sample fixation or probe hybridization with the Fixed RNA Profiling Assay?
- Can the Enhancer be kept at 42°C or 65°C for longer periods of time?
- Is the Fixed RNA Profiling assay compatible with intracellular staining?
- Which thermal cyclers are compatible with the Fixed RNA Profiling assay?