Software
- How does Single Cell Flex data generated from GEM-X chips compare to Flex data generated from NextGEM chips?
- Cell Ranger detected a lower number of cells than expected in my FFPE samples with the Fixed RNA Profiling (Flex) assay. Can I change cell calling parameters to recover more cells?
- Why do you recommend a T nucleotide at the 25th position of custom probes in the Technical Note for custom probe design for Flex and Visium assays?
- How do I analyze CRISPR screening + Single Cell Gene Expression Flex data in Cell Ranger?
- Is trajectory analysis compatible with the Fixed RNA Profiling (Flex) assay?
- How can I get FASTQ files by sample for a multiplexed FRP library?
- How does Fixed RNA Profiling (Flex) data compare to 3' Gene Expression (GEX) data?
- Is it expected to see a significant background signal from genomic DNA in Chromium Fixed RNA Profiling data?
- What does the term fold of coverage mean in the probe-based assays?
- Is it possible to integrate fixed RNA profiling data with 3' or 5' gene expression data?
- Can I use PDX/xenograft tissue with Flex Gene Expression?
- Does Cell Ranger normalize UMI counts based on the coverage of genes in Fixed RNA Profiling?
- How do I use Cell Ranger aggr to aggregate Fixed RNA Profiling datasets?