Chromium Nuclei Isolation Kit
- When should I start the 10 minute lysis timer for the Nuclei Isolation Kit?
- How can I improve the yield of my nuclei for hard-to-dissociate tissues such as heart, muscle, and fibrous tissue using the Chromium Nuclei Isolation Kit?
- How can I optimize my nuclei suspension if I am using an untested tissue type in the Chromium Nuclei Isolation kit?
- How can I reduce myelin debris from brain in the Chromium Nuclei Isolation kit?
- What should I do if I have >5% of my nuclei suspension staining live?
- What if I do not get enough nuclei from 50mg of tissue using the Chromium Nuclei Isolation Kit?
- Can I isolate nuclei from OCT embedded samples using the Chromium Nuclei Isolation Kit?
- Is there enough RNase Inhibitor to complete 16 samples in the Chromium Nuclei Isolation Kit?
- What sample types are compatible with the Chromium Nuclei Isolation Kit?
- What if my tissue is not on the list of tissues tested with the Chromium Nuclei Isolation Kit?
- How does the Chromium Nuclei Isolation Kit work?
- What are some advantages of the Chromium Nuclei Isolation Kit over other nuclei isolation methods?
- What Chromium Nuclei Isolation Kit configuration should I use?
- What 10x assays are compatible with the Chromium Nuclei Isolation Kit?
- Will the nuclei isolated using the Chromium Nuclei Isolation Kit be compatible with Feature Barcode Technology (Cell Surface Protein, CellPlex, CRISPR Screening), or V(D)J profiling?
- Is the Chromium Nuclei Isolation Kit compatible with fresh tissues?
- How can I improve my nuclei recovery using the Chromium Nuclei Isolation kit?
- Do I need to sort to remove debris after isolating nuclei using the Chromium Nuclei Isolation kit?
- Are protocol modifications recommended when using the Chromium Nuclei Isolation Kit with poor quality or RNase-rich tissues?
- I want to do a test run with my sample using the Chromium Nuclei Isolation Kit, do I need to use RNase Inhibitor?
- Why did my Nuclei Isolation Column clog during centrifugation?
- How does the cell data compare to nuclei data?
- How do I know when my tissue is fully dissociated using the Chromium Nuclei Isolation Kit?
- What are the best practices for tissue dissociation using the Chromium Nuclei Isolation Kit?
- Can an alternative RNase Inhibitor be used with the Chromium Nuclei Isolation Kit?
- Can I purchase the RNase Inhibitor found in the Chromium Nuclei Isolation kit?
- Do I need to run a lysis timeline using the Chromium Nuclei Isolation kit?
- How should I count nuclei after isolating nuclei using the Chromium Nuclei Isolation Kit?
- Are there differences in the Chromium Nuclei Isolation protocol when isolating nuclei for Single Cell Gene Expression applications, Single Cell Multiome ATAC + GEX, and Single Cell ATAC?
- Do I need to vortex my nuclei to dissociate aggregates during the Chromium Nuclei Isolation workflow?