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Q&A NEW CONTACT SUPPORT
  1. 10X Genomics
  2. General Single Cell RNA-seq
  3. High Throughput Single Cell Gene Expression + Single Cell Immune Profiling

High Throughput Single Cell Gene Expression + Single Cell Immune Profiling

  • What is the expected liquid volume in each well after a successful HT run?
  • What dry baths are supported for use with HT?
  • Are there differences in microfluidic failure rates (clogs and wetting failures) between the standard and HT chips?
  • How do I assemble HT Gel Beads in the HT Gel Bead holder?
  • What comes in my HT kit?
  • Why do I have a 16 reaction library preparation kit with my 8 reaction HT kit?
  • What are the best practices for HT Dynabead cleanups?
  • When do I need to purchase a Library Construction Kit for HT?
  • How many Feature Barcode kits do I need for the HT assay?
  • Are HT reagents and consumables cross-compatible with reagents and consumables from our standard assays?
  • How are HT gel beads and standard gel beads different?
  • If I have a wetting failure / clog in one of the sample wells of the HT assay, will this impact the other paired sample?
  • What should I do if I have an odd number of samples for HT?
  • Why is the HT multiplet rate lower than our standard assay?
  • What is the sensitivity of the Single Cell HT products?
  • What is the minimum number of samples I can run on an HT chip?
  • What sample preparation recommendations should be considered when using the HT solution?
  • Is cell counting different for the Next GEM Single Cell HT assay?
  • How can we generate a larger GEM volume and recover 2x the amount of cells in the same amount of time in the HT assay compared to our standard assays?
  • What is the cell capture rate of the HT products?
  • What is the minimum/maximum number of cells that I can recover per channel without CellPlex for the HT assay?
  • Are Chip M/Chip N available for purchase separately?
  • What assays will be available in HT?
  • Should I proceed if there is an HT assay sample clog?
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