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  2. Visium Spatial Gene Expression for FFPE Assays
  3. Visium Spatial Gene Expression for FFPE
  4. Staining

Staining

Content related to the Visium for FFPE Staining Demonstrated Protocols

  • Can I use Xylene alternatives for Deparaffinization with Visium for FFPE v1?
  • How often should I refresh my deparaffinization and H&E staining solutions? 
  • How should I store the Gene Expression Slide after coverslip removal with Visium for FFPE?
  • What steps can I take to minimize the impact of autofluorescence during immunofluorescence staining?
  • Is Visium for FFPE compatible with immunohistochemistry (IHC)?
  • How long is my FFPE tissue stable after coverslip removal?
  • Can immunofluorescence staining and imaging have a negative impact on Visium for FFPE v1 assay sensitivity?
  • Does the Visium for FFPE v1 Immunofluorescence Demonstrated Protocol differ from standard immunofluorescence protocols?
  • What reagents are needed to perform the Visium for FFPE v1 IF Staining Demonstrated Protocol?
  • What reagents are needed to perform the Visium for FFPE v1 H&E Staining Demonstrated Protocol?
  • How long is my FFPE tissue stable after H&E staining and coverslipping?
  • Is my antibody compatible with the Visium for FFPE v1 Immunofluorescence (IF) Staining Demonstrated Protocol?
  • Is the Visium for FFPE Immunofluorescence Staining Demonstrated Protocol compatible with indirect immunofluorescence?
  • Do I need to re-optimize my antibodies for the Visium for FFPE v1 with Immunofluorescence Demonstrated Protocol?
  • How do I decrosslink my tissue before the Visium for FFPE v1 assay?
  • I can’t purchase the recommended TE Buffer, pH 9.0, are there any alternatives that could be used?
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