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  1. 10X Genomics
  2. Visium Spatial Gene Expression for FFPE Assays
  3. Visium Spatial Gene Expression for FFPE
  4. Tissue Preparation

Tissue Preparation

Content related to fixation, embedding, and information included in the Visium for FFPE Tissue Preparation Guide

  • Is the Slide Reset Demonstrated Protocol (CG000332) compatible with the Visium Spatial Gene Expression for FFPE assay? 
  • How does over-fixation impact Visium for FFPE v1 assay performance?
  • How are DV200% and Visium for FFPE assay performance correlated?
  • Why does Visium for FFPE v1 call for DV200 as opposed to RIN evaluation for sample quality assessment?
  • Why are there so many drying steps in the Visium for FFPE v1 Demonstrated Protocols?
  • Should I punch and re-embed my FFPE block so my tissue can fit within the Capture Area?
  • Can I place FFPE sections onto a Visium Spatial Gene Expression v1 Slide during multiple sectioning sessions?
  • What should I do if my FFPE tissue has detached from the Visium Gene Expression v1 Slide?
  • How well does tissue adhere to Visium Slides with Visium for FFPE v1 compared to other tissue-based assays?
  • I don't have a desiccator, what can I use instead?
  • How do I transport my Visium Spatial Gene Expression v1 Slides after the FFPE tissue has been stained and imaged?
  • How should I fix and embed my tissue for Visium for FFPE? 
  • Should I generate FFPE blocks from fresh tissue to use with the Visium for FFPE assay? 
  • How do I practice sectioning and tissue placement for Visium for FFPE v1?
  • Where can I purchase control FFPE tissue blocks for practicing sectioning and placement?
  • How do I transport my Visium Spatial Gene Expression FFPE v1 Slides to another facility when tissue has already been placed?
  • I need to transport my FFPE tissue block to another facility, how should I do this?
  • How should I store my FFPE tissue blocks?
  • What tissue section thickness should I use for the Visium for FFPE v1 assay? 
  • How do I determine if my tissue block will be compatible with the Visium for FFPE v1 assay?
  • How do I study a large tissue specimen (greater than 6.5 mm x 6.5 mm) using Visium for FFPE v1?
  • When using different FFPE blocks, what is the best way to section and prepare the Visium Gene Expression v1 Slide with tissue?
  • Can I section from the same FFPE tissue block across multiple days?
  • Do I need to decontaminate the microtome between different tissue types?
  • How can I easily visualize the Fiducial Frame on the Visium Slide during tissue section placement?
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