Sample Preparation
Content related to fixation, embedding, and information included in the Visium for FFPE Tissue Preparation Guide
- Is the Slide Reset Demonstrated Protocol (CG000332) compatible with the Visium Spatial Gene Expression for FFPE assay?
- How does over-fixation impact Visium for FFPE assay performance?
- How are DV200% and Visium for FFPE assay performance correlated?
- Why does Visium for FFPE call for DV200 as opposed to RIN evaluation for sample quality assessment?
- Why are there so many drying steps in the Visium for FFPE Demonstrated Protocols?
- Should I punch and re-embed my FFPE block so my tissue can fit within the Capture Area?
- Can I place FFPE sections onto a Visium Spatial Gene Expression Slide during multiple sectioning sessions?
- What should I do if my FFPE tissue has detached from the Visium Gene Expression Slide?
- How well does tissue adhere to Visium Slides with Visium for FFPE compared to other tissue-based assays?
- I don't have a desiccator, what can I use instead?
- How do I transport my Visium Spatial Gene Expression Slides after the FFPE tissue has been stained and imaged?
- How should I fix and embed my tissue for Visium for FFPE?
- Should I generate FFPE blocks from fresh tissue to use with the Visium for FFPE assay?
- How do I practice sectioning and tissue placement for Visium for FFPE?
- Where can I purchase control FFPE tissue blocks for practicing sectioning and placement?
- How do I transport my Visium Spatial Gene Expression Slides to another facility when tissue has already been placed?
- I need to transport my FFPE tissue block to another facility, how should I do this?
- How should I store my FFPE tissue blocks?
- What tissue section thickness should I use for the Visium for FFPE assay?
- How do I determine if my tissue block will be compatible with the Visium for FFPE assay?
- How do I study a large tissue specimen (greater than 6.5 mm x 6.5 mm) using Visium for FFPE?
- When using different FFPE blocks, what is the best way to section and prepare the Visium Gene Expression Slide with tissue?
- Can I section from the same FFPE tissue block across multiple days?
- Do I need to decontaminate the microtome between different tissue types?
- How can I easily visualize the Fiducial Frame on the Visium Slide during tissue section placement?