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  1. 10X Genomics
  2. Visium for FFPE
  3. Sample Preparation

Sample Preparation

Content related to fixation, embedding, and information included in the Visium for FFPE Tissue Preparation Guide

  • Is the Slide Reset Demonstrated Protocol (CG000332) compatible with the Visium Spatial Gene Expression for FFPE assay? 
  • How does over-fixation impact Visium for FFPE assay performance?
  • How are DV200% and Visium for FFPE assay performance correlated?
  • Why does Visium for FFPE call for DV200 as opposed to RIN evaluation for sample quality assessment?
  • Why are there so many drying steps in the Visium for FFPE Demonstrated Protocols?
  • Should I punch and re-embed my FFPE block so my tissue can fit within the Capture Area?
  • Can I place FFPE sections onto a Visium Spatial Gene Expression Slide during multiple sectioning sessions?
  • What should I do if my FFPE tissue has detached from the Visium Gene Expression Slide?
  • How well does tissue adhere to Visium Slides with Visium for FFPE compared to other tissue-based assays?
  • I don't have a desiccator, what can I use instead?
  • How do I transport my Visium Spatial Gene Expression Slides after the FFPE tissue has been stained and imaged?
  • How should I fix and embed my tissue for Visium for FFPE? 
  • Should I generate FFPE blocks from fresh tissue to use with the Visium for FFPE assay? 
  • How do I practice sectioning and tissue placement for Visium for FFPE?
  • Where can I purchase control FFPE tissue blocks for practicing sectioning and placement?
  • How do I transport my Visium Spatial Gene Expression Slides to another facility when tissue has already been placed?
  • I need to transport my FFPE tissue block to another facility, how should I do this?
  • How should I store my FFPE tissue blocks?
  • What tissue section thickness should I use for the Visium for FFPE assay? 
  • How do I determine if my tissue block will be compatible with the Visium for FFPE assay?
  • How do I study a large tissue specimen (greater than 6.5 mm x 6.5 mm) using Visium for FFPE?
  • When using different FFPE blocks, what is the best way to section and prepare the Visium Gene Expression Slide with tissue?
  • Can I section from the same FFPE tissue block across multiple days?
  • Do I need to decontaminate the microtome between different tissue types?
  • How can I easily visualize the Fiducial Frame on the Visium Slide during tissue section placement?
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