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  3. Visium for FFPE - Protocol Steps

Visium for FFPE - Protocol Steps

Content related to steps detailed in the Visium for FFPE User Guide

  • Why does my Visium Spatial Gene Expression for FFPE library look unexpected or have low yield?
  • Why is my Visium Spatial Gene Expression for FFPE Cq value for Cycle Number Determination so high?
  • How do I interpret the qPCR plot to determine the Sample Index PCR cycle number?
  • Can I use an alternative to the KAPA SYBR Fast qPCR Master Mix (KK4600) for Cycle Number Determination during the Visium and Visium for FFPE Gene Expression workflows?
  • During qPCR for Cycle Number Determination of the Visium and Visium for FFPE Gene Expression workflows, what should the real time controller lid temperature be set to?
  • Can I use the Dual Index Kit Plate TT Set A, 96 rxns (PN-1000215) for Sample Index PCR with Visium for FFPE?
  • What does a good Visium Spatial Gene Expression for FFPE library look like?
  • What is the structure of the final Visium for FFPE library?
  • What Dual Index Plate is compatible with Visium for FFPE?
  • Can I use alternative methods to qPCR such as Nanodrop or Qubit for Cycle Number Determination during the Visium for FFPE Gene Expression workflow?
  • What are the stopping points in the Visium for FFPE workflow?
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