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  1. 10X Genomics
  2. Visium Spatial Gene Expression for FFPE Assays
  3. Visium Spatial Gene Expression for FFPE
  4. Protocol Steps

Protocol Steps

Content related to steps detailed in the Visium Spatial Gene Expression for FFPE User Guide

  • Why are the "Reads Mapped Confidently to the Probe Set" or "Reads Mapped Confidently to the Filtered Probe Set" metrics in my Spatial Gene Expression web summary file low?
  • Why does my Visium Spatial Gene Expression for FFPE v1 library look unexpected or have low yield?
  • Why is my Visium Spatial Gene Expression for FFPE v1 Cq value for Cycle Number Determination so high?
  • How do I interpret the qPCR plot to determine the Sample Index PCR cycle number?
  • During qPCR for Cycle Number Determination of the Visium and Visium for FFPE Gene Expression workflows, what should the real time controller lid temperature be set to?
  • Can I use the Dual Index Kit Plate TT Set A, 96 rxns (PN-1000215) for Sample Index PCR with Visium for FFPE?
  • What does a good Visium Spatial Gene Expression for FFPE v1 library look like?
  • What is the structure of the final Visium for FFPE v1 library?
  • What assays is the Dual Index Kit TS Set A (PN-1000251) compatible with?
  • Can I use alternative methods to qPCR such as Nanodrop or Qubit for Cycle Number Determination during the Visium for FFPE v1 Gene Expression workflow?
  • What are the stopping points in the Visium for FFPE v1 workflow?
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