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  1. 10X Genomics
  2. Spatial Gene Expression for FFPE
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General

General articles pertaining to Visium Spatial Gene Expression for FFPE

  • Which thermal cyclers are compatible with the Visium for FFPE v1 assay?
  • I'm using the Thermo Fisher Scientific Veriti© 96-Well Thermal Cycler (Cat No. 4375786) to run Visium, how should I set up the thermal cycler to maximize success?
  • Are tissue microarrays (TMAs) supported with Visium for FFPE v1?
  • My thermal cycler lid temperature doesn't go lower than 50°C, what should I do?
  • Can I perform Spatial Gene Expression analysis using older FFPE blocks?
  • What is the purpose of the Tissue Adhesion Test and how should it be performed?
  • What is the difference between formaldehyde, formalin, and paraformaldehyde?
  • What fixation and embedding methods are compatible with Visium for FFPE assays?
  • What sample handling methods can be implemented to minimize the likelihood of FFPE tissue detachment on Visium Slides?
  • Can I use snap frozen tissue with the Visium for FFPE assay?
  • Does Visium Spatial Gene Expression for FFPE require a Tissue Optimization experiment?
  • How many Slide Seals are required for the Staining Demonstrated Protocols and Visium for FFPE v1 workflow?
  • How many reactions are in the large Transcriptome Probe Kit and FFPE Reagent Kit?
  • How many reactions are in the small Transcriptome Probe Kit and FFPE v1 Reagent Kit?
  • What Visium for fresh-frozen 10x supplied consumables are shared with Visium for FFPE v1?
  • Can I run different tissue types on a single Visium Spatial Gene Expression Slide for the Visium for FFPE v1 assay?
  • What tissue types can be used with the Visium Spatial Gene Expression for FFPE solution?
  • What Visium for FFPE v1 Kits are required for generating libraries?
  • How many cells are captured in a single spot for FFPE tissues?
  • How can I avoid mRNA degradation during sample preparation and slide processing for Visium for FFPE?
  • Do we recommend replicates for Visium for FFPE v1?
  • Do the Visium slides have the same charge as the SuperFrost Plus or other slides with similar charge coating?
  • What is Visium Spatial Gene Expression for FFPE, and how does it work?
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