V(D)J Amplification
- How to use custom V(D)J primers on Chromium Connect?
- What parts of the V(D)J amplification workflow are included on the Chromium Connect?
- Does the V(D)J assay begin with cells or cDNA? What is the cDNA volume that is loaded onto the instrument?
- What parts of the V(D)J amplification workflow on the Chromium Connect require manual intervention?
- When running the V(D)J amplification assay, do I have to run 8 samples at a time, or can I just run one or two?
- Do I need to reload anything before running the 5’ V(D)J amplification assay?
- Which species are supported for V(D)J Amplification?
- Are my V(D)J amplification reagents safe at room temperature overnight or over the weekend?
- Do I have to take my V(D)J final libraries off the deck immediately after the run is complete?