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  1. 10X Genomics
  2. General Single Cell RNA-seq
  3. Feature Barcoding - Cell Multiplexing

Feature Barcoding - Cell Multiplexing

  • What are the differences between the tube-based and plate-based CMO labeling protocols for using the 3' CellPlex kit for Cell Multiplexing?
  • Can antibody labeling be performed after CMO labeling when using the 3' CellPlex kit for Cell Multiplexing?
  • Can I perform shallow sequencing on 3’ Cell Multiplexing libraries to assess the quality of my CellPlex data?
  • How does high background from unbound CMOs affect Cell Multiplexing metrics in 3’ CellPlex experiments?
  • How can I reduce background from unbound CMOs using the 3’ CellPlex kit for Cell Multiplexing?
  • Which nuclei isolation protocols are supported for use with the 3' CellPlex Kit for Cell Multiplexing?
  • What is the labeling efficiency when using the 3’ CellPlex kit for Cell Multiplexing?
  • Which buffers can be used for the wash steps post CMO labeling when using the 3’ CellPlex kit for Cell Multiplexing?
  • Can I reduce the volume of CMO used for labeling when using the 3’ CellPlex kit for Cell Multiplexing?
  • Is MULTI-Seq the same as the 3’ CellPlex kit for Cell Multiplexing?
  • Are microbeads such as the Miltenyi Dead Cell Removal Kit compatible with the 3’ CellPlex Kit for Cell Multiplexing?
  • What resources are available for the 3' CellPlex Kit for Cell Multiplexing?
  • What affects the cell multiplet rate and cell multiplet filtering when using the 3' CellPlex Kit for Cell Multiplexing?
  • Which 10x Genomics assays are compatible with the 3' CellPlex Kit for Cell Multiplexing?
  • What sample types are compatible with the 3' CellPlex Kit for Cell Multiplexing?
  • Can cell multiplets be filtered out when using the 3’ CellPlex Kit for Cell Multiplexing?
  • Is flow cytometry compatible with the 3’ CellPlex Kit for Cell Multiplexing?
  • How many cell multiplets will remain undetected in my final data when using the 3' CellPlex Kit for Cell Multiplexing?
  • What is the recommended number of CMO tags when using the 3’ CellPlex Kit for Cell Multiplexing?
  • Can I use Capture Sequence 2 for both Cell Multiplexing and CRISPR Screening libraries?
  • Are targeted cell recoveries greater than 10,000 cells supported using standard throughput kits?
  • After CMO-labeling and pooling, how long can cells or nuclei sit before loading onto the chip?
  • Are methanol-fixed cells compatible with the 3' CellPlex Kit for Cell Multiplexing?
  • Are cryopreserved samples compatible with the 3' CellPlex Kit for Cell Multiplexing?
  • Is the rate of clogs or wetting failures higher when using the 3' CellPlex Kit for Cell Multiplexing?
  • How many cells do I need for CMO labeling when using the 3' CellPlex Kit for Cell Multiplexing?
  • What sample pooling ratios are supported when using the 3’ CellPlex Kit for Cell Multiplexing?
  • Why was the part number updated for the 3’ Feature Barcode kit?
  • Can I pool cells and nuclei when using the the 3’ CellPlex Kit for Cell Multiplexing?
  • Can I use more than one CMO tag per sample when using the 3’ CellPlex Kit for Cell Multiplexing?
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