- How do I determine the average fragment size of a Single Cell ATAC or Multiome ATAC final library?
- Should I optimize transposition times for ATAC v1.1, v2 or Multiome assays?
- Can I purchase nuclei buffer for my ATAC or Multiome experiment?
- Why do I see an increased High Molecular Weight peak at 2000bp in the Multiome ATAC library trace?
- What is the purpose of the quenching reaction?
- Does the transposition reaction damage mRNA or promote nuclei leakage?
- How are the ATAC and RNA fragments separated to generate individual Multiome ATAC and GEX libraries?
- Do I need to generate both Multiome ATAC and Multiome Gene Expression libraries? Or can I just perform one or the other?