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  • How do I determine the average fragment size of a Single Cell ATAC or Multiome ATAC final library?
  • Should I optimize transposition times for ATAC v1.1, v2 or Multiome assays?
  • Can I purchase nuclei buffer for my ATAC or Multiome experiment?
  • Why do I see an increased High Molecular Weight peak at 2000bp in the Multiome ATAC library trace?
  • What is the purpose of the quenching reaction?
  • Does the transposition reaction damage mRNA or promote nuclei leakage?
  • How are the ATAC and RNA fragments separated to generate individual Multiome ATAC and GEX libraries?
  • Do I need to generate both Multiome ATAC and Multiome Gene Expression libraries? Or can I just perform one or the other?
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