Nuclei Isolation
- How do I prepare and store 10% IGEPAL CA-630 and IGEPAL CO-360 stock solutions for nuclei isolations?
- Which nuclei isolation protocol should I use for the Single Cell Multiome ATAC + Gene Expression assay?
- How much residual membrane is acceptable when preparing nuclei for the Single Cell ATAC or Single Cell Multiome ATAC + Gene Expression assays?
- What are the differences in the nuclei isolation protocols among the 3', standalone ATAC and Multiome (ATAC+GEX) assays?
- What cleanup steps are compatible with my Multiome sample?
- Can I store the Diluted Nuclei Buffer, Lysis Buffers or Wash Buffers used in the Multiome assay?
- How do I perform a lysis timeline to optimize my nuclei isolation for Single Cell Multiome ATAC + Gene Expression?
- What are the best practices for nuclei isolation for Single Cell Multiome ATAC and Gene Expression?
- Can I use an alternative RNase inhibitor part number?