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Q&A NEW CONTACT SUPPORT
  1. 10X Genomics
  2. Single Cell Multiome ATAC + Gene Expression
  3. Nuclei Isolation

Nuclei Isolation

  • How do I prepare and store 10% IGEPAL CA-630 and IGEPAL CO-360 stock solutions for nuclei isolations?
  • Which nuclei isolation protocol should I use for the Single Cell Multiome ATAC + Gene Expression assay?
  • How much residual membrane is acceptable when preparing nuclei for the Single Cell ATAC or Single Cell Multiome ATAC + Gene Expression assays?
  • What are the differences in the nuclei isolation protocols among the 3', standalone ATAC and Multiome (ATAC+GEX) assays?
  • What cleanup steps are compatible with my Multiome sample?
  • Can I store the Diluted Nuclei Buffer, Lysis Buffers or Wash Buffers used in the Multiome assay?
  • How do I perform a lysis timeline to optimize my nuclei isolation for Single Cell Multiome ATAC + Gene Expression?
  • What are the best practices for nuclei isolation for Single Cell Multiome ATAC and Gene Expression?
  • Can I use an alternative RNase inhibitor part number?
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