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Q&A NEW CONTACT SUPPORT
  1. 10X Genomics
  2. Single Cell Gene Expression
  3. Automated Single Cell Gene Expression with Chromium Connect

Automated Single Cell Gene Expression with Chromium Connect

  • How do I create a troubleshooting package from the Chromium Connect for Support?
  • How often do I need a Preventative Maintenance (PM) performed on the Chromium Connect?
  •  How long does Daily/Weekly maintenance of the Chromium Connect take?
  • How often do I need to perform maintenance on my Chromium Connect system?
  • What is included in the optional pooling step on the Chromium Connect?
  • How much volume is used for library dilutions during the KAPA qPCR setup on the Chromium Connect?
  • What is included in the optional qPCR on the Chromium Connect?
  • Can we run feature barcoding off the remaining Chromium Connect cDNA?
  • How much of my cDNA is used for library prep on the Chromium Connect?
  • Can the Chromium Connect start after the post GEM-RT step?
  • What happens when I have a wetting/clog failure on the Chromium Connect?
  • Are there any safe stopping points in the Chromium Connect workflow?
  • Are my Chromium Connect reagents safe at room temperature overnight or over the weekend?
  • What is the sample volume that is loaded onto the Chromium Connect instrument?
  • Do I have to run 8 samples at a time on the Chromium Connect, or can I just run one or two?
  • What parts of the workflow on the Chromium Connect require manual intervention?
  •  How do I integrate my data from the Chromium Connect into LIMS?
  • How do I enter sample information for my experiment on the Chromium Connect?
  • Can manual Single Cell 3’ reagents be used with the Chromium Connect?
  • Can the Chromium Connect be modified for use with other assays?
  • How does the data from the Chromium Connect compare to the manual assay?
  • What instruction-level should I choose when I set up my Chromium Connect run?
  •  Can the Chromium Connect instrument be used by multiple users?
  • What are the levels of cross-contamination on the Chromium Connect?
  • Does the Chromium Connect have a UV lamp?
  • Does the Chromium Connect have a HEPA filter? 
  • Is there a recommendation to place the Chromium Connect in a pre-amp or post-amp room?
  • What are the weight/dimensions and power requirements needed for the Chromium Connect instrument?
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