Tissue Fixation and Staining
- Is the Visium with Immunofluorescence Demonstrated Protocol compatible with the Targeted Gene Expression assay?
- I have already optimized my antibodies for immunofluorescence on its own. Do I need to re-optimize my antibodies for the Visium with Immunofluorescence assay?
- How much time does the Visium with Immunofluorescence Demonstrated Protocol require?
- Does the Immunofluorescence portion of the 10x Demonstrated Protocol differ from standard Immunofluorescence protocols?
- Is my antibody compatible with the Visium Immunofluorescence (IF) Staining Demonstrated Protocol?
- What is the maximum number of antibodies that can be used with the demonstrated protocol for Visium with Immunofluorescence Staining ?
- What tissue types have been validated with the Visium with Immunofluorescence Demonstrated Protocol?
- What sample preparation methods are compatible with Visium with Immunofluorescence (IF)?
- Can I perform both H&E and Immunofluorescence staining on the same tissue section?
- If I have performed Visium with H&E staining, do I need to repeat tissue optimization with Immunofluorescence (IF) staining?
- What should I do if tissue remains on the Visium Tissue Optimization slide after the tissue removal step?
- Can the H&E staining process have a negative impact on RNA integrity and reduce assay sensitivity?
- Can staining methods other than H&E be used with the Visium Solution?
- Is Visium compatible with cells or tissues expressing a reporter allele such as GFP?
- How are the tissue sections fixed to the capture areas on the Visium slide?