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  1. 10X Genomics
  2. Visium
  3. Tissue Fixation and Staining

Tissue Fixation and Staining

  • I have already optimized my antibodies for immunofluorescence on its own. Do I need to re-optimize my antibodies for the Visium for fresh-frozen Immunofluorescence assay?
  • How much time does the Immunofluorescence Staining Demonstrated Protocol for Visium for fresh-frozen require? 
  • Does the Visium for fresh-frozen Immunofluorescence Demonstrated Protocol differ from standard immunofluorescence protocols?
  • Is my antibody compatible with the Visium for fresh-frozen Immunofluorescence (IF) Staining Demonstrated Protocol?
  • What is the maximum number of antibodies that can be used when performing Visium with Immunofluorescence?
  • What tissue types have been validated with the Visium for fresh-frozen Immunofluorescence Demonstrated Protocol?
  • What sample preparation methods are compatible with the Visium for fresh-frozen Immunofluorescence (IF) demonstrated protocol?
  • Can I perform both H&E and Immunofluorescence staining on the same tissue section?
  • If I have performed Visium with H&E staining, do I need to repeat tissue optimization with Immunofluorescence (IF) staining?
  • What should I do if tissue remains on the Visium Tissue Optimization slide after the tissue removal step?
  • Can the H&E staining process have a negative impact on RNA integrity and reduce assay sensitivity?
  • Can staining methods other than H&E be used with the Visium Solution?
  • Is Visium for fresh-frozen compatible with cells or tissues expressing a reporter allele such as GFP?
  • How are the tissue sections fixed to the capture areas on the Visium slide?
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