Tissue Fixation and Staining
- What types of samples have we validated with the Visium with Immunofluorescence Demonstrated Protocol?
- What sample preparation type can I use with the Visium with Immunofluorescence Demonstrated protocol?
- Can I perform both H&E and Immunofluorescence with Visium on the same section?
- If I have performed Visium with H&E staining, do I need to repeat tissue optimization with Immunofluorescence (IF) staining?
- Can the H&E staining process have a negative impact on RNA integrity and reduce assay sensitivity?
- Can staining methods other than H&E be used with the Visium Solution?
- Is Visium compatible with cells or tissues expressing a reporter allele such as GFP?
- How are the tissue sections fixed to the capture areas on the Visium slide?