Tissue Fixation and Staining
- I have already optimized my antibodies for immunofluorescence on its own. Do I need to re-optimize my antibodies for the Visium for fresh-frozen Immunofluorescence assay?
- How much time does the Immunofluorescence Staining Demonstrated Protocol for Visium for fresh-frozen require?
- Does the Visium for fresh-frozen Immunofluorescence Demonstrated Protocol differ from standard immunofluorescence protocols?
- Is my antibody compatible with the Visium for fresh-frozen Immunofluorescence (IF) Staining Demonstrated Protocol?
- What is the maximum number of antibodies that can be used when performing Visium with Immunofluorescence?
- What tissue types have been validated with the Visium for fresh-frozen Immunofluorescence Demonstrated Protocol?
- What sample preparation methods are compatible with the Visium for fresh-frozen Immunofluorescence (IF) demonstrated protocol?
- Can I perform both H&E and Immunofluorescence staining on the same tissue section?
- If I have performed Visium with H&E staining, do I need to repeat tissue optimization with Immunofluorescence (IF) staining?
- What should I do if tissue remains on the Visium Tissue Optimization slide after the tissue removal step?
- Can the H&E staining process have a negative impact on RNA integrity and reduce assay sensitivity?
- Can staining methods other than H&E be used with the Visium Solution?
- Is Visium for fresh-frozen compatible with cells or tissues expressing a reporter allele such as GFP?
- How are the tissue sections fixed to the capture areas on the Visium slide?