.
Logo
  • PRODUCTS
  • TECHNOLOGY
  • COMPANY
  • CAREERS
  • Support
  • COMMUNITY
  • Sign in
  1. Support
  2. Q&A
Q&A NEW CONTACT SUPPORT
  1. 10X Genomics
  2. Visium
  3. Sample Preparation

Sample Preparation

  • Are fresh-frozen tissues containing bone compatible with Visium Spatial Gene Expression?
  • Can I perform both Single Cell Gene Expression and Visium Spatial Gene Expression experiments on my OCT-embedded tissue block?
  • Can OCT affect bulk RNA extraction and RIN assessment?
  • How can I more easily visualize the fiducial frame during tissue section placement?
  • Can I section from the same tissue block across multiple days?
  • Can OCT cover the Fiducial Frame or extend outside the capture area?
  • Do I need to decontaminate the cryostat between different tissues types?
  • How can I prevent tissue sections from freeze-drying during cryostat sectioning?
  • Is there a maximum tissue section thickness for the Visium for fresh-frozen assay?
  • What is OCT?
  • If I am running a time course study collecting tissues, what is the best way to section and prepare the Visium Gene Expression slide with tissue?
  • Can my tissue cover the fiducial frame on a Visium slide?
  • Are tissues flash-frozen in liquid nitrogen compatible with Visium?
  • How should I prepare my slide with tissue for shipment?
  • How do I study a large tissue specimen (greater than 6.5 mm x 6.5 mm) using Visium for fresh-frozen?
  • What tissue types have been tested with the Visium solution?
  • Is Visium compatible with cell culture?
  • How should I prepare mouse eye tissue for the Visium assay?
  • How do I snap freeze and embed my tissue in OCT?
  • Can I run multiple tissue sections in one capture area of the Visium Slide?
  •  Is it possible that the permeabilization time may differ between cancer types with the Visium solution?
  • What tissue types have been optimized with Visium?
  • What fresh-frozen tissue section thickness should I place on Visium Slides?
  • Are fixed tissues compatible with Visium for fresh-frozen?
  • Is Visium compatible with immunohistochemistry (IHC)?
  • How many tissue sections can be tested on a Visium slide?
footer-logo

Biology at True Resolution

About
  • Solutions
  • Technology
  • Company
  • Careers
  • Support
  • Community
  • Contact Us
Legal Notices
  • Privacy Policy
  • Terms of Use
  • Email Preferences
  • Other Legal Notices
Resources
  • Publications
  • Applications
  • Videos
Headquarters
  • +1 925 401 7300
  • +1 800 709 1208
  • info@10xgenomics.com
Social
©2018 10x Genomics. All Rights Reserved.
Sign up for product and event updates