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Q&A NEW CONTACT SUPPORT
  1. 10X Genomics
  2. Spatial Gene Expression for Fresh Frozen
  3. General

General

  • Are the Visium slides charged?
  • Are the Visium Spatial Tissue Optimization or Gene Expression slides re-usable if not all sections are used?
  • At what step in the workflow can I use the Visium Spatial Slide Reset Demonstrated Protocol?
  • Can I run different tissue types on a single Visium Spatial Gene Expression Slide for the Visium for fresh-frozen assay?
  • Can I use different permeabilization time points on a single Visium Gene Expression slide?
  • Can I use the Visium Spatial Slide Reset Demonstrated Protocol to practice section placement for Visium?
  • Do the spatial barcodes differ across individual capture areas on the Visium Spatial Gene Expression Slide?
  • Do we recommend running replicate tissue sections with Visium for fresh-frozen?
  • Does 10x recommend a model system to use as a control for practicing on prior to using a real sample with Visium?
  • How can I avoid mRNA degradation during sample preparation and slide processing for Visium for fresh-frozen?
  • How do I determine the optimal permeabilization time from my Visium Tissue Optimization (TO) experiment?
  • How does the Visium Spatial Slide Reset Demonstrated Protocol affect subsequent assay performance?
  • How long does it take to perform the Visium Spatial Slide Reset Demonstrated Protocol?
  • How many cells are captured in a single spot?
  • How many libraries are generated from the Visium Spatial Gene Expression slide?
  • How many oligos are contained in each spot?
  • How many sections will be removed if I perform the Visium Spatial Slide Reset Demonstrated Protocol?
  • How many spots are within a single capture area on the Visium v1 Spatial Gene Expression Slide?
  • How many times can I reset a Visium slide?
  • How much space is there between spots (referred to as white space)?
  • How should I store the Imaging Test Slide found in the Visium Accessory Kit?
  • How should I store Visium Slides once tissue sections have been placed?
  • How should unused Visium slides be stored?
  • Is a cDNA footprint signal in my Tissue Optimization experiment negative control with IF staining expected?
  • Is there detectable mRNA transcript mislocalization under the tissue with Visium for fresh-frozen?
  • What 10x reagents do I need to purchase to perform the Visium Spatial Slide Reset Demonstrated Protocol?
  • What are best practices for assembling and handling the Visium Slide Cassette?
  • What are the main reasons to use the Visium with Immunofluorescence Demonstrated Protocol?
  • What are the storage conditions of slides reset with the Visium Spatial Slide Reset Demonstrated Protocol?
  • What if tissue remains on the slide after performing the Visium Spatial Slide Reset Demonstrated Protocol?
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