Feature Barcoding - CRISPR Screening
- Troubleshooting CRISPR issues on low fraction of guide reads and high fraction of unrecognized protospacers
- Can you generate CRISPR Screening libraries with the GEM-X Universal 3’ Gene Expression v4 assay?
- Which 10x Genomics Single Cell CRISPR approach should I use?
- Are nuclei samples compatible with CRISPR screening?
- Why does my CRISPR Screening library trace have additional peaks?
- Which Capture Sequence and sgRNA integration site should I use for 3' CRISPR Screening experiments?
- Do you recommend enriching for transduced cells?
- What should I consider when designing my 3' or 5' CRISPR pool?
- What is MOI and how do I assess it?
- What resources are available for my CRISPR experimental design?
- Is Feature Barcode Technology for CRISPR Screening compatible with non-human species?