Sample Prep - Nuclei
- What is the highest spin speed and spin time recommended for nuclei isolation for Single Cell ATAC or Single Cell Multiome assays?
- Is there a nuclei isolation protocol for ATAC in plants?
- I'm having trouble accurately counting nuclei. What can I do?
- Can I sort nuclei for Single Cell ATAC sequencing or Single Cell Multiome ATAC + GEX?
- How do I perform a lysis timeline to optimize my nuclei isolation for Single Cell ATAC sequencing?
- How can I optimize my nuclei prep for Single Cell ATAC sequencing?
- Can I run samples with low cell viability for ATAC or Multiome assays?
- Do I need to dissociate tissues into single cells first before isolating nuclei, or can I isolate nuclei directly from the whole tissue?
- Why is using diluted Nuclei Buffer for resuspending my nuclei important?