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  1. 10X Genomics
  2. Single Cell ATAC
  3. Sample Prep - Nuclei

Sample Prep - Nuclei

  • Which nuclei isolation protocol should I use for the Single Cell ATAC assay?
  • What is the highest spin speed and spin time recommended for nuclei isolation for Single Cell ATAC or Single Cell Multiome assays?
  • Is there a nuclei isolation protocol for ATAC in plants?
  • I'm having trouble accurately counting nuclei. What can I do?
  • Can I sort nuclei for Single Cell ATAC sequencing or Single Cell Multiome ATAC + GEX?
  • How do I perform a lysis timeline to optimize my nuclei isolation for Single Cell ATAC sequencing?
  • How can I optimize my nuclei prep for Single Cell ATAC sequencing?
  • Can I run samples with low cell viability for ATAC or Multiome assays?
  • Do I need to dissociate tissues into single cells first before isolating nuclei, or can I isolate nuclei directly from the whole tissue?
  • Why is using diluted Nuclei Buffer for resuspending my nuclei important?
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