Sequencing
- What concentration should I load my libraries at for sequencing?
- What is 'read pairs per cell' and how does it relate to sequencer yield?
- Which Illumina SBS kit should I select for sequencing?
- Can I perform shallow sequencing to assess the quality of Single Cell 3' Gene Expression libraries?
- Are index sequences shared across dual index plates?
- Is nucleotide diversity in the index read important for Illumina sequencing?
- Can single index and dual index libraries be pooled for sequencing?
- Can I use the 100-cycle NovaSeq kit for Single Cell 3' and 5' assays?
- Why do some sequence reads contain the TSO oligo at the beginning of Read 2?
- What Illumina sequencers are supported for Single Cell 3' libraries?