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GEM Generation & Barcoding

  • Is it expected to see a large pellet of lyophilized Template Switch Oligo B (PN-2001027) used with Chromium GEM-X Single Cell 3’ v4? 
  • Are Next GEM reagents cross-compatible with non-Next GEM reagents?
  • How can the poly-A based cDNA synthesis work on RNA from isolated nuclei?
  • What fraction of mRNA transcripts are captured per cell?
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