Sequencing
- Which 10x Genomics library types are compatible with sequencing on Ultima Genomics platforms?
- What considerations are there for sequencing 10x Genomics libraries on NextSeq 1000/2000 Sequencing Systems with XLEAP SBS kits?
- What index combinations should I select when sequencing low plex pools of 10x Genomics Libraries generated with the Dual Index TT or Dual Index TS plates?
- Why has the loading concentration on the NovaSeq 6000 Illumina sequencing platform been updated for Next GEM library types?
- Which 10x Genomics library types are compatible with sequencing on the Element AVITI™ System?
- What sequencing configuration should I use?
- How to setup a 10x Genomics sample sheet in Illumina's BaseSpace for demultiplexing
- What considerations are there for sequencing 10x Genomics libraries on NovaSeq X Series instruments?
- Can I sequence my 10x Genomics libraries with Singular Genomics G4 Sequencing Platform?
- How do alternative quantification methods other than qPCR impact library loading?
- Which 10x Genomics assays are compatible with long-read sequencing applications?
- Can I detect alternative transcript isoforms using 10x assays?
- I stored my GEM-RT Products, cDNA, and/or final library for longer than recommended in the User Guide. Can I use them to complete the protocol for sequencing?
- Should I increase the number of reads per cell if I increase the number of antibodies?
- Do I need custom sequencing primers for 10x libraries?
- Why are there unequal ratios of the four oligos (or oligo dropout) in my 10x library after sequencing?
- How do you set up a NextSeq run on BaseSpace?
- What oligos are in my sample index?
- Is a sample sheet required for sequencing?
- Is the Sample Index plate compatible across products?
- Is the Sample Index PCR required if not multiplexing samples?