Sequencing
- Which 10x Assays are compatible with long-read sequencing applications?
- Can I detect alternative transcript isoforms using 10x assays?
- I stored my GEM-RT Products, cDNA, and/or final library for longer than recommended in the User Guide. Can I use them to complete the protocol for sequencing?
- Should I increase the number of reads per cell if I increase the number of antibodies?
- Do I need custom sequencing primers for 10x libraries?
- What is the recommended amount of PhiX to spike into 10x libraries?
- Why are there unequal ratios of the four oligos (or oligo dropout) in my 10x library after sequencing?
- How do you set up a NextSeq run on BaseSpace?
- What oligos are in my sample index?
- Is a sample sheet required for sequencing?
- Is the Sample Index plate compatible across products?
- Is the Sample Index PCR required if not multiplexing samples?