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  1. 10X Genomics
  2. General
  3. Reagents

Reagents

  • If there are shipment delays, is it acceptable to use Dynabeads™ MyOne™ SILANE that have been stored at room temperature or frozen?
  • How are the ratios of SPRIselect beads calculated in 10x assays?
  • What are the best practices for using SPRIselect beads in 10x assays?
  • How many freeze thaw cycles are the gel beads stable for?
  • Why is it critical to load the wells in the specified order?
  • Is there a plate magnetic separator recommended by 10x?
  • Can I mix reagents from different kits?
  • What is the shelf life of 10x reagents?
  • Can I pool leftover gel beads?
  • Will failure in one channel affect samples in other channels?
  • Can AMPure beads be used instead of SPRIselect beads?
  • What is the importance of emulsion-safe plastic consumables?
  • What is surrogate fluid?
  • Can Silane DynaBeads be substituted with other beads?
  • Can I modify enzymes or other buffer components?
  • Can gel beads, slides, chips, and other reagents be used past their expiration dates?
  • Can Gel Beads be stored at temperatures other than -80°C?
  • Can Gel Beads be aliquoted into individual tubes?
  • What is the fill volume on reagents?
  • Differences in partitioning oil appearance
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