Reagents
- Why is it critical to load the wells in the specified order?
- What is the maximum number of thaw-freeze cycles for the i7 sample index plate?
- Can I mix reagents from different kits?
- What is the shelf life of 10x reagents?
- Can I pool leftover gel beads?
- Will failure in one channel affect samples in other channels?
- Can AMPure beads be used instead of SPRIselect beads?
- Importance of emulsion-safe plastic consumables
- How many freeze-thaws is Additive A stable for?
- What is surrogate fluid?
- Can Silane DynaBeads be substituted with other beads?
- Can I modify enzymes or other buffer components?
- Can library and gel bead kits be used past their expiration dates?
- Can Gel beads be stored at temperatures other than -80C?
- Can Gel Beads be aliquoted into individual tubes?
- What is the fill volume on reagents?
- Differences in partitioning oil appearance