Sequencing
- How do I pool 10x libraries for Illumina sequencing?
- Should I select Workflow A or Workflow B for the i5 index sequence?
- Can I sequence longer than 91-98bp on Read 2 for Single Cell 3' and 5' Gene Expression libraries?
- Can I decrease the sequencing length of Read 2 for Single Cell Gene Expression assays?
- Why do I see high levels of Malat1 in my gene expression data?
- What is the difference between Single Cell 3' and 5’ Gene Expression libraries?
- What is sequencing saturation?
- How much sequencing saturation should I aim for?
- What is the recommended sequencing depth for Single Cell 3' and 5' Gene Expression libraries?