Library Construction
- What options are there for performing a targeted enrichment for my gene expression libraries?
- If full length mRNA is transcribed, how is the assay biased to 3' or 5'?
- Are 10x Single Cell gene expression libraries strand-specific?
- What are the additional peaks in my Single Cell Gene Expression library?
- Is there a safe stopping point between Post-Ligation Cleanup and SI-PCR?
- Is there a sequence preference during cDNA fragmentation?
- Can ERCC spike-ins be used for normalization?