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  2. General Single Cell RNA-seq
  3. Library Construction

Library Construction

  • What options are there for performing a targeted enrichment for my gene expression libraries?
  • If full length mRNA is transcribed, how is the assay biased to 3' or 5'?
  • Are 10x Single Cell gene expression libraries strand-specific?
  • What are the additional peaks in my Single Cell Gene Expression library?
  • Is there a safe stopping point between Post-Ligation Cleanup and SI-PCR?
  • Is there a sequence preference during cDNA fragmentation?
  • Can ERCC spike-ins be used for normalization?
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