Post GEM-RT Cleanup & cDNA Amplification
- Can I store purified cDNA beyond 1 week at -20°C?
- Why is my total cDNA yield low?
- Why is the aqueous layer cloudy after breaking GEMs?
- What types of RNA can be detected?
- What are the additional peaks in my post-cDNA amplification QC?
- What is the expected size range for amplified cDNA?
- Why is the recovery agent not completely removed after breaking emulsions?