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  1. 10X Genomics
  2. General Single Cell RNA-seq
  3. GEM Generation & Barcoding

GEM Generation & Barcoding

  • How should I take photographs to document clogs and wetting failures with GEM-X applications?
  • What are the largest cell types tested with Chromium Single Cell assays?
  • Are GEM-X Chips more robust to emulsion failure than Next GEM Chips? 
  • Is it normal to observe Partitioning Oil at the bottom of the pipette tip after collecting GEMs?
  • How should I take photographs to document clogs and wetting failures?
  • Is it possible to capture microRNAs in the Gene Expression assays?
  • What is a template switch oligo (TSO)?
  • What is the maximum number of cells that can be profiled?
  • Should I proceed if there is a sample clog?
  • How often do multiple Gel Beads end up in a partition?
  • Can GEMs be stored after isothermal incubation?
  • Can GEMs be stored after the partitioning of single cells?
  • What is a wetting failure and how can they be recognized?
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