GEM Generation & Barcoding
- How can I test whether my cells can be partitioned into GEMs?
- Is it possible to capture microRNAs in the Gene Expression assays?
- Can you run Single Cell 5' and 3' samples on the same Single Cell A Chip?
- What is a template switch oligo (TSO)?
- What is the maximum number of cells that can be profiled?
- Recommended plastics for GEM-RT reaction
- Is a heat sealer necessary to seal the plate after GEM recovery?
- What are the properties of the Gel Bead-in-Emulsion (GEMs)?
- Should I proceed if there is a sample clog?
- How often do multiple Gel Beads end up in a partition?
- Can GEMs be stored after isothermal incubation?
- Can GEMs be stored after the partitioning of single cells?
- What is a wetting failure and how can they be recognized?